Produced:Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Recombinant:Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Protease Inhibitor:Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Mutagenesis:Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Software:Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
Article Title: Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DYKDDDDK Tag Antibody (anti-FLAG) from rabbit Cell Signaling Cat#2368 Anti-GFP from mouse Roche Cat#11814460001 Monoclonal Anti-a-Tubulin antibody produced in mouse Sigma-Aldrich Cat#T6074 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Cat#18-8817-33 Rabbit TrueBlot: Anti-Rabbit IgG HRP Rockland Cat#18-8816-33 Chemicals, Peptides, and Recombinant Proteins Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Protease inhibitor cocktail Sigma-Aldrich Cat#5056489001 Benzonase Nuclease Merck Cat#70746-3 FRET-labeled mononucleosomes Greg Bowman Lab N/A FAM-labeled mononucleosomes Greg Bowman Lab N/A Human PARP1 Enzo Life Science Cat#ALX-201-063-C020 32P-b-NAD Perkin Elmer Cat#NEG023X Phosphate sensor Thermo Fisher Scientific CatPV4406 Human PARP1 Thermo Fisher Scientific Cat#11040H08B50 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#78430 DSS Creative Molecules Cat#001S Human PARP1 Trevigen Cat#4668-500-01 Human PARP1 BPS Bioscience Cat#BPS-80501 99.8% D2O ACROS Cat#AC16630 Deposited Data Mendeley Dataset This paper https://doi.org/10.17632/hm7zy8kn94.1 Experimental Models: Cell Lines Human: U2OS cells Francis Crick Cell Services Cat#2179 Experimental Models: Organisms/Strains E. coli Rosetta 2 (DE3) Novagen Cat#71400 Oligonucleotides DNA oligonucleotides for mutagenesis This paper Table S2 Recombinant DNA Human ALC1fl (16-879) pNIC-CH2 This paper N/A YFP-ALC1 pDEST-YFP/FRT/TO Ahel et al., 2009 N/A Software and Algorithms I-Tasser server Yang et al., 2015 https://zhanglab.ccmb.med.umich.edu/I-TASSER/ GROMACS software package Berendsen et al., 1995 http://www.gromacs.org ATSAS software package Petoukhov et al., 2012 https://www.embl-hamburg.de/biosaxs/ software.html MotionCorr Li et al., 2013 http://cryoem.ucsf.edu/software/driftcorr.html MotionCor2 Zheng et al., 2017 http://msg.ucsf.edu/em/software/motioncor2.html RELION Scheres, 2012, 2015 https://www2.mrc-lmb.cam.ac.uk/relion/index.php/ Download_%26_install Gctf Zhang, 2016 http://www.mrc-lmb.cam.ac.uk/kzhang/ (Continued on next page) e1 Molecular Cell 68, 847–859.e1–e7, December 7, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER xQuest/xProphet software pipeline Walzthoeni et al., 2012 http://proteomics.ethz.ch/cgi-bin/xquest2_cgi/ index.cgi DynamX HDX Data Analysis Software Waters Cat#720005145en Other 5 mL HisTrap FF GE Healthcare Cat#17525501 HiPrep 26/10 Desalting GE Healthcare Cat#17508701 5 mL HiTrap Q HP GE Healthcare Cat#17115401 5 mL HiTrap SP HP GE Healthcare Cat#17115201 HiLoad 16/600 Superdex 200 pg GE Healthcare Cat#28989335 Superdex 200 10/300 GL (discontinued) GE Healthcare Cat#17517501 Gel Filtration Markers Kit Sigma-Aldrich Cat#MWGF1000 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 GFP-Trap_A Chromotek Cat#gta-20 Bio SEC-3 Agilent Cat#5190-6854 Ni-NTA Agarose resin QIAGEN Cat#30210 Enzymate BEH pepsin column Waters Cat#186007233 ACQUITY UPLC BEH C18 VanGuard Pre-column Waters Cat#186003975 ACQUITY UPLC BEH C18 Column Waters Cat#186002350
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